SPEECH ENHANCEMENT BASED ON SPARSE THEORY UNDER NOISY ENVIRONMENT

[[abstract]]Recently, the sparse algorithm for sparse enhancement is more and more popular issues. In this paper, we classify the process of the sparse theory to enhance speech signal into two parts, one is for dictionary training part and the other is signal reconstruction part. We focus on the White Gaussian Noise. Clean speech dictionary D is trained by K-SVD algorithm. The orthogonal matching pursuit(OMP) algorithm is used to obtain the sparse coefficients X of clean speech dictionary D. Denoising performance of the experiments shows that our proposed method is superior than other methods in SNR, LLR, SNRseg and PESQ.[[sponsorship]]National Taipei University[[conferencetype]]國際[[conferencedate]]20150718~20150719[[booktype]]電子版[[iscallforpapers]]Y[[conferencelocation]]Tokyo, Japa

3D FACE MODEL CONSTRUCTION BASED ON KINECT FOR FACE RECOGNITION

[[abstract]]We propose a simpler and faster method to recognize face. First, we use Kinect to detect frontal face and get depth image information with face, then we portrayed face in OpenGL to construct a three-dimensional face model based on the depth information. The face model also retains texture information of the original face images, and to create a complete change depth of face. It has a good result of repairing the distortion in side face. We can get a set face images with different angles by the method proposed, In recognition part, we use PCA(Principal Component Analysis) to reduce the dimensions, and classified with SVM(Support Vector Machine). The experiments show that the side face recognition can have good results.[[sponsorship]]National Taipei University[[conferencetype]]國際[[conferencedate]]20150718~20150719[[booktype]]電子版[[iscallforpapers]]Y[[conferencelocation]]Tokyo, Japa

AMPK- mediated formation of stress granules is required for dietary restriction- induced longevity in Caenorhabditis elegans

Stress granules (SGs) are nonmembranous organelles that are dynamically assembled and disassembled in response to various stressors. Under stressed conditions, polyadenylated mRNAs and translation factors are sequestrated in SGs to promote global repression of protein synthesis. It has been previously demonstrated that SG formation enhances cell survival and stress resistance. However, the physiological role of SGs in organismal aging and longevity regulation remains unclear. In this study, we used TIAR- 1::GFP and GTBP- 1::GFP as markers to monitor the formation of SGs in Caenorhabditis elegans. We found that, in addition to acute heat stress, SG formation could also be triggered by dietary changes, such as starvation and dietary restriction (DR). We found that HSF- 1 is required for the SG formation in response to acute heat shock and starvation but not DR, whereas the AMPK- eEF2K signaling is required for starvation and DR- induced SG formation but not heat shock. Moreover, our data suggest that this AMPK- eEF2K pathway- mediated SG formation is required for lifespan extension by DR, but dispensable for the longevity by reduced insulin/IGF- 1 signaling. Collectively, our findings unveil a novel role of SG formation in DR- induced longevity.In addition to heat stress, starvation and dietary restriction (DR) can activate stress granule (SG) formation in Caenorhabditis elegans. HSF- 1 and AMPK are two key regulators for the SG formations. HSF- 1 is required for the SG formation in response to acute heat shock and starvation but not DR, whereas the AMPK- eEF2K pathway is required for starvation and DR- induced SG formation but not heat shock. Furthermore, AMPK- mediated SG formation contributes to DR- induced longevity.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/1/acel13157-sup-0008-Figurelegends.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/2/acel13157-sup-0001-FigS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/3/acel13157-sup-0006-TableS1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/4/acel13157-sup-0007-TableS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/5/acel13157-sup-0005-FigS5.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/6/acel13157-sup-0003-FigS3.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/7/acel13157.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/8/acel13157-sup-0002-FigS2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/9/acel13157-sup-0004-FigS4.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/155936/10/acel13157_am.pd

Celecoxib extends C. elegans lifespan via inhibition of insulin‐like signaling but not cyclooxygenase‐2 activity

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/86911/1/ACEL_688_sm_FigS1-S2-TableS1-S2.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/86911/2/j.1474-9726.2011.00688.x.pd

Investigating the Effect of Color Mask on Sensitivity for the Color Schlieren Imaging

This work aims to investigate influence of the color mask for color schlieren technique. To demonstrate the effect, a series of experiments by changing the color mask shape are carried out. The applied color mask is a round mask with three RGB (red, green, and blue) colors gaps which is first presented by Settles, G. S. in 1980. In this work, experiments used a digital color mask instead of a film mask. The applied digital color mask is prepared in computer and projected by an LCD projector. The major discussed parameter is ratio of the outside and inside mask diameters. With the new technique, the digital color mask can be easily varied in computer directly and projected conveniently. In this work, the best color mask with outside diameter ca. 10 mm and inside diameter ca. 8 mm that has the highest sensitivity for color schlieren imaging has been successfully found. In general, the larger inside diameter, the higher sensitivity. The inside diameter has the limitation while the passing light intensity is too weak

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

[[abstract]]The cytotoxicity of berberine on C6 rat glioma cells indicated that berberine induced morphological changes and caused cell death through G2/M arrest and apoptosis. While undergoing apoptosis, there was a remarkable accumulation of G2/M cells with the upregulatoin of Weel but it also inhibited cyclin B, CDK1 and Cdc25c that led to G2/M arrest. Along with cytotoxicity in C6 cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9, -3 and -8 and DNA fragmentation were induced. Berberine increased the levels of GADD153 and GRP 78 in C6 cells based on the examination of Western blotting and this is a major hallmark of endoplasmic reticulum (ER) stress. We also found that berberine promoted the production of reactive oxygen species and Ca2+ in C6 cells. Western blotting assay also showed that berberine inhibited the levels of anti-apoptotic protein Bcl-2 but increased the levels of pro-apoptotic protein Bax before leading to a decrease in the levels of mitochondrial membrane potential (Delta psi(m)) followed by cytochrome c release that caused the activations of capase-9 and -3 for apoptotic occurrence. The caspase-8, -9 and -3 were activated by berberine in C6 cells based on the substrate solution (PhiPhiLux-G(1)D(1), CaspaLux 8-L1D2, CaspaLux 9-M1D2 for caspase-3, -8 and -9, respectively) and analyzed by flow cytometer and each inhibitor of caspase-8, -9 and -3 led to increase the percentage of viable C6 cells after exposure to berberine. This finding was also confirmed by Western blot assay which showed that berberine promoted the active form of caspase-8, -9 and -3. These results demonstrate that the cytotoxicity of berberine in C6 rat glioma cells is attributable to apoptosis mainly through induced G2/M-arrested cells, in an ER-dependent manner, via a mitochondria-dependent caspase pathway regulated by Bax and Bcl-2